ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (1): 130-137.doi: 10.11843/j.issn.0366-6964.2015.01.017

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Ampicillin Residue Depletion in Chicken Tissues

SUN Li-rui1,2，XIE Xing 3，XIE Kai-zhou1,2*，ZHANG Xiao-jie1,2，WANG Jian-feng1,2，CUI Lu-lu1,2，LIU Jian-yu1,2，ZHANG Gen-xi 1,2，DAI Guo-jun1,2，WANG Jin-yu1,2   

  1. (1.College of Animal Science and Technology，Yangzhou University，Yangzhou 225009，China；2.Key Laboratory for Animal Genetic，Breeding，Reproduction and Molecular Design of Jiangsu Province，Yangzhou 225009，China；3.College of Veterinary Medicine，Nanjing Agricultural University，Nanjing 210095，China)
  • Received:2014-04-30 Online:2015-01-23 Published:2015-01-23

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Abstract:

 A study on ampicillin (AMP) residue depletion was conducted in chicken tissues.AMP was extracted from chicken tissues with sodium dihydrogen phosphate solution.After being deproteinizated with acetonitrile and degreased in n-hexane saturated，the analyte was extracted by saturated dichloromethane.The supernatant was reacted with formaldehyde under acidic and boiling conditions.Finally，AMP was determinated by high performance liquid chromatography (HPLC) with fluorescence detector.The excitation wavelength was set at 327 nm and emission wavelength was set at 409 nm.The average recovery in chicken tissue was 75.31%-85.87% with coefficients of variation (CV) lower than 10.20%.The limit of detection (LOD) was 3.5 μg•kg－1 (S/N=3) and the limit of quantitation (LOQ） was 10.0 μg•kg－1(S/N=10).After the chickens were orally administered successively AMP of 120 mg•kg－1 and 240 mg•kg-1 of body weight one time every day for 7 days，the maximum residues of AMP in chicken tissues were detected at 4h after final administration.Concentrations of AMP fell sharply at the 3th withdrawal day，all lower than MRL (50 μg•kg－1) at the 5th withdrawal day，and all lower than LOD (5.0 μg•kg－1) at the 9th withdrawal day.At the same time definition，the concentration of AMP in muscle was lowest，but highest in liver.However，AMP in kidney needed more time for depletion.The residues of AMP in chicken tissues were all positively correlated with AMP orally administered doses.The concentration-time dates were analyzed by WT1.4 software to get the withdrawal time (WT) with 95% upper tolerance limit，the WT advised in normal dose group was 4 d，and in double dose group was 5 d.

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